Forensic Application of Monoclonal Anti-Human Glycophorin A Antibody in Samples from Decomposed Bodies to Establish Vitality of the Injuries. A Preliminary Experimental Study

Glycophorins are an important group of red blood cell (RBC) transmembrane proteins. Monoclonal antibodies against GPA employed in immunohistochemical staining during post-mortem examination: Through this method, it is possible to point out the RBC presence tissues. This experimental study aims investigate anti-GPA order evaluate vitality lesion from corpses different decomposition state. Six cases were selected, analyzing autopsies’ documentation performed by Institute Legal Medicine Rome 2010–2018: four samples fractured bones and three soft For control case, fracture region femur was sampled. The results present confirm preliminary other studies, remarking importance highlight signs survival. Moreover, suggests that use technique should be routinely applied with advanced putrefaction phenomena, even when radiological investigation performed, macroscopic inconclusive, H&E not reliable. application demonstrated monoclonal antibody anti-human on bone fractures tissues could verify whether vital or not.